Research

GE Healthcare DeltaVision OMX 3D-SIM

Master
Content

The DeltaVision OMX is a 3D structured illumination microscope (3D-SIM) designed to provide a highly stable, multi-channel imaging platform for advanced microscopy applications. The design of the microscope is such that super-resolution 3D images may be rapidly obtained and constructed. Live or fixed cells may be imaged.

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Key Features

Media Component
The DeltaVision OMX is an advanced, multimode super-resolution microscope system offering the Blaze SIM Module for dynamic, high speed 3D-SIM.
Content
  • Multiple illumination modalities
    • Structured illumination 3D SIM
    • Advanced TIRF and pK/pA module
    • Localization imaging
    • Wide-field imaging
  • SIM Blaze module for full 3D super-resolution reconstruction
  • UltimateFocus laser-based hardware autofocus technology
  • "Pan and scan" function
  • High precision motor-driven stage

Cameras

  • PCO front illuminated Edge sCMOS x 2
  • 5-bit, 5.5Mp
  • 512x512 for 3D-SIM
  • 1024x1024 for wide-field and TIRF. High speed, low light sensitivity

Acquisition Software

  • OMX Master Control Workstation (Windows 7)

Workstation

  • softWoRx®/Imaris (CentOS v6, Linux)

Filter sets

  • 2x 8 position filter wheels

Lasers

  • 405nm ± 2
  • 488nm ± 2; 568nm ± 1
  • 642nm ± 2. 4-channel simultaneous imaging capability

Objectives:

  • 60X oil PLANPON6, NA 1.42 (required for use with sCMOS camera)
  • 60X APON, NA 1.49 with correction collar (required for TIRF)

Illumination

  • 6-color InsightSSI solid state wide-field illumination. Supports DAPI/CFP/GFP, FITC/YFP/Alexa Fluor™ 568, mCherry/Cy™5/DIC.