Research

Protein and Monoclonal Antibody Production Core

Master
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About the Core

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The protein and monoclonal antibody production core is one of the shared resources at Baylor College of Medicine main campus.

The core services including:

  • Monoclonal Antibody Production
  • Recombinant Protein Production (Expression and Purification)
  • Protein Characterization
     

The core provides recombinant protein production in E.coli, insect and mammalian systems and has had more than 30 years of excellent experience in producing new mouse monoclonal antibodies using proteins, synthetic peptides and subcellular fraction as antigen.

New protein characterization services is open for users in 2022!

All core services can be requested in iLab.

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Getting Started

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If you have questions and plan to make use of the core, please email atc-protein-mab@bcm.edu. We can offer consultation in Zoom or in-person meeting.

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Recombinant Protein Expression

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The core has large refrigerated shaking incubators and bioreactors to overexpress recombinant proteins of interest in E.coli, insect and mammalian systems.

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Protein and Antibody Chromatography Purification

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The core implements chromatography techniques to purify protein and Mab produced in different expression systems. Quality control and biophysical characterization of purified proteins and MAbs can be performed, including endotoxin and nucleases testing, analytical gel filtration, and Thermofluor/DSF assays.

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Mouse Monoclonal Antibody Production

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The core can generate custom mouse monoclonal antibodies (MAbs), including those targeted to proteins, synthetic peptides and subcellular fractions.

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Protein Characterization

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The core providea various protein characterization technologies and instruments. Instruments are available in the core to characterize proteins and other macromolecules.

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