Tsz-Kwong Man, Ph.D., B.S.
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Positions
- Associate Professor
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Pediatrics-Oncology
Baylor College of Medicine
Houston, TX US
- Member
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Dan L Duncan Comprehensive Cancer Center
Baylor College of Medicine
Houston, Texas United States
Education
- Postdoctoral Training at Washington University
- 01/2001 - St. Louis, MO United States
- PhD from University Of Texas Medical School At Houston
- 01/1997 - Houston, Texas United States
- BSc from Hong Kong Baptist College
- 01/1986 - Hong Kong, Hong Kong
Professional Interests
- Research Interests
Professional Statement
Molecular classification of cancer using a systems biology approach. We use a systems biology approach to generate genomic and proteomic profiles from various specimens of cancer patients, such as tumors and plasma. The goal of our laboratory is to integrate these diverse data by various classification algorithms to construct prediction models, which can better predict clinical phenotypes of pediatric cancer, such as drug resistance, metastatic potential, and prognosis. Ultimately, we aim to develop an easy-to-use and minimally invasive blood test that can help to diagnose and prognosticate patients with pediatric cancers, so they can be treated as early and precisely as possible. Mechanistic study of metastatic determinants. Using a proteomic approach, we have identified p27 (CDKN1B) as a crucial metastatic determinant in pediatric osteosarcoma. We have shown that p27 is frequently mislocalized in the cytoplasm of osteosarcoma cases, and the mislocalization increases the metastatic potential of tumor cells. p27 is a well-known tumor suppressor gene that regulates normal cell cycle progression when it is located in the nucleus, but its oncongenic function when located in the cytoplasm is still largely unknown. It may act as a master switch between tumorigenesis and cancer progression. We use a panel of targeted genomic and proteomic methods to dissect the mechanism of the cytoplasmic p27 in the promotion of metastasis in osteosarcoma and other cancers. Our goal is to test if cytoplasmic p27 can be used as a novel therapeutic target to abolish metastasis in cancer. Cancer bioinformatics. With the explosion of the genomic data and the decreasing cost of the genomic assays, there is a wealth of genomic data available in various public repositories. These resources provide an unprecedented opportunity for data mining to identify common cancer signatures and mechanisms in various cancers. Understanding these common features of different types of cancer will provide an important clue on how to target cancer in general. Our lab is interested in developing bioinformatic methods and tools that can utilize these resources to answer specific biological or clinical questions that will lead to a better understanding of common cancer phenotypes and the development of novel therapeutic targets.Websites
Link to VIICTR
Selected Publications
- Flores RJ, Li Y, Yu A, Shen J, Rao PH, Lau SS, Vannucci M, Lau CC, Man TK "A systems biology approach reveals common metastatic pathways in osteosarcoma.." BMC Syst Biol. 2012;6:50. Pubmed PMID: 22640921
- Egler RA, Li Y, Dang TA, Peters TL, Leung E, Huang S, Russell HV, Liu H, Man TK "An integrated proteomic approach to identifying circulating biomarkers in high-risk neuroblastoma and their potential in relapse monitoring.." Proteomics Clin Appl. 2011 Oct;5(41527):532-41. Pubmed PMID: 21833997
- Li Y, Flores R, Yu A, Okcu MF, Murray J, Chintagumpala M, Hicks J, Lau CC, Man TK "Elevated expression of CXC chemokines in pediatric osteosarcoma patients.." Cancer. 2011 Jan 1;117(1):207-17. Pubmed PMID: 20806347
- Li Y, Dang TA, Shen J, Hicks J, Chintagumpala M, Lau CC, Man TK "Plasma proteome predicts chemotherapy response in osteosarcoma patients.." Oncol. Rep.. 2011 Feb;25(2):303-14. Pubmed PMID: 21165584
- Guo B, Villagran A, Vannucci M, Wang J, Davis C, Man TK, Lau C, Guerra R "Bayesian estimation of genomic copy number with single nucleotide polymorphism genotyping arrays.." BMC Res Notes. 2010;3:350. Pubmed PMID: 21192799
- Cruz-Marcelo A, Guerra R, Vannucci M, Li Y, Lau CC, Man TK "Comparison of algorithms for pre-processing of SELDI-TOF mass spectrometry data.." Bioinformatics. 2008 Oct 1;24(19):2129-36. Pubmed PMID: 18694894
- Daves MH, Hilsenbeck SG, Lau CC, Man TK "Meta-analysis of multiple microarray datasets reveals a common gene signature of metastasis in solid tumors.." BMC Med Genomics. 2011;4:56. Pubmed PMID: 21736749
- Li Y, Dang TA, Shen J, Perlaky L, Hicks J, Murray J, Meyer W, Chintagumpala M, Lau CC, Man TK "Identification of a plasma proteomic signature to distinguish pediatric osteosarcoma from benign osteochondroma.." Proteomics. 2006 Jun;6(11):3426-35. Pubmed PMID: 16673437
- Yu L, Baxter PA, Voicu H, Gurusiddappa S, Zhao Y, Adesina A, Man TK, Shu Q, Zhang YJ, Zhao XM, Su JM, Perlaky L, Dauser R, Chintagumpala M, Lau CC, Blaney SM, Rao PH, Leung HC, Li XN "A clinically relevant orthotopic xenograft model of ependymoma that maintains the genomic signature of the primary tumor and preserves cancer stem cells in vivo.." Neuro-oncology. 2010 Jun;12(6):580-94. Pubmed PMID: 20511191
- Wong KK, Tsang YT, Shen J, Cheng RS, Chang YM, Man TK, Lau CC "Allelic imbalance analysis by high-density single-nucleotide polymorphic allele (SNP) array with whole genome amplified DNA.." Nucleic Acids Res.. 2004;32(9):e69. Pubmed PMID: 15148342
- Allen CE, Li L, Peters TL, Leung HC, Yu A, Man TK, Gurusiddappa S, Phillips MT, Hicks MJ, Gaikwad A, Merad M, McClain KL "Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells.." J. Immunol.. 2010 Apr 15;184(8):4557-67. Pubmed PMID: 20220088
- Gu JX, Wei MY, Rao PH, Lau CC, Behl S, Man TK "CGI: Java Software for Mapping and Visualizing Data from Array-based Comparative Genomic Hybridization and Expression Profiling.." Gene Regul Syst Bio. 2007;1:131-6. Pubmed PMID: 19936083
- Dang TA, Man TK "Classification of sarcomas using bioinformatics and molecular profiling.." Curr Pharm Biotechnol. 2007 Apr;8(2):83-91. Pubmed PMID: 17430156
- Rabin KR, Man TK, Yu A, Folsom MR, Zhao YJ, Rao PH, Plon SE, Naeem RC "Clinical utility of array comparative genomic hybridization for detection of chromosomal abnormalities in pediatric acute lymphoblastic leukemia.." Pediatr Blood Cancer. 2008 Aug;51(2):171-7. Pubmed PMID: 18253961
- Cheung AN, Srivastava G, Pittaluga S, Man TK, Ngan H, Collins RJ "Expression of c-myc and c-fms oncogenes in trophoblastic cells in hydatidiform mole and normal human placenta.." J. Clin. Pathol.. 1993 Mar;46(3):204-7. Pubmed PMID: 8463412
- Cheung AN, Srivastava G, Chung LP, Ngan HY, Man TK, Liu YT, Chen WZ, Collins RJ, Wong LC, Ma HK "Expression of the p53 gene in trophoblastic cells in hydatidiform moles and normal human placentas.." J Reprod Med. 1994 Mar;39(3):223-7. Pubmed PMID: 8035377
- Man TK, Chintagumpala M, Visvanathan J, Shen J, Perlaky L, Hicks J, Johnson M, Davino N, Murray J, Helman L, Meyer W, Triche T, Wong KK, Lau CC "Expression profiles of osteosarcoma that can predict response to chemotherapy.." Cancer Res.. 2005 Sep 15;65(18):8142-50. Pubmed PMID: 16166288
- Man TK, Lu XY, Jaeweon K, Perlaky L, Harris CP, Shah S, Ladanyi M, Gorlick R, Lau CC, Rao PH "Genome-wide array comparative genomic hybridization analysis reveals distinct amplifications in osteosarcoma.." BMC Cancer. 2004 Aug 7;4:45. Pubmed PMID: 15298715
- Yang Y, Tsui HC, Man TK, Winkler ME "Identification and function of the pdxY gene, which encodes a novel pyridoxal kinase involved in the salvage pathway of pyridoxal 5'-phosphate biosynthesis in Escherichia coli K-12.." J. Bacteriol.. 1998 Apr;180(7):1814-21. Pubmed PMID: 9537380
- Rainusso N, Man TK, Lau CC, Hicks J, Shen JJ, Yu A, Wang LL, Rosen JM "Identification and gene expression profiling of tumor-initiating cells isolated from human osteosarcoma cell lines in an orthotopic mouse model.." Cancer Biol. Ther.. 2011 Aug 15;12(4):278-87. Pubmed PMID: 21617384
- Yang Y, Zhao G, Man TK, Winkler ME "Involvement of the gapA- and epd (gapB)-encoded dehydrogenases in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.." J. Bacteriol.. 1998 Aug;180(16):4294-9. Pubmed PMID: 9696782
- Man TK, Zhao G, Winkler ME "Isolation of a pdxJ point mutation that bypasses the requirement for the PdxH oxidase in pyridoxal 5' -phosphate coenzyme biosynthesis in Escherichia coli K-12.." J. Bacteriol.. 1996 Apr;178(8):2445-9. Pubmed PMID: 8636054
- Man TK, Pease AJ, Winkler ME "Maximization of transcription of the serC (pdxF)-aroA multifunctional operon by antagonistic effects of the cyclic AMP (cAMP) receptor protein-cAMP complex and Lrp global regulators of Escherichia coli K-12.." J. Bacteriol.. 1997 Jun;179(11):3458-69. Pubmed PMID: 9171388
- De Bortoli M, Castellino RC, Lu XY, Deyo J, Sturla LM, Adesina AM, Perlaky L, Pomeroy SL, Lau CC, Man TK, Rao PH, Kim JY "Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8.." BMC Cancer. 2006;6:223. Pubmed PMID: 16968546
- Ma O, Cai WW, Zender L, Dayaram T, Shen J, Herron AJ, Lowe SW, Man TK, Lau CC, Donehower LA "MMP13, Birc2 (cIAP1), and Birc3 (cIAP2), amplified on chromosome 9, collaborate with p53 deficiency in mouse osteosarcoma progression.." Cancer Res.. 2009 Mar 15;69(6):2559-67. Pubmed PMID: 19276372
- Man TK, Stormo GD "Non-independence of Mnt repressor-operator interaction determined by a new quantitative multiple fluorescence relative affinity (QuMFRA) assay.." Nucleic Acids Res.. 2001 Jun 15;29(12):2471-8. Pubmed PMID: 11410653
- Man TK, Li Y, Dang TA, Shen J, Perlaky L, Lau CC "Optimising the use of TRIzol-extracted proteins in surface enhanced laser desorption/ ionization (SELDI) analysis.." Proteome Sci. 2006;4:3. Pubmed PMID: 16556310
- De Bortoli M, Castellino RC, Skapura DG, Shen JJ, Su JM, Russell HV, Hicks MJ, Man TK, Kim JY "Patched haploinsufficient mouse rhabdomyosarcoma overexpress secreted phosphoprotein 1 and matrix metalloproteinases.." Eur. J. Cancer. 2007 May;43(8):1308-17. Pubmed PMID: 17467979
- Rabin K, Man TK, Lau CC "Personalized care of pediatric cancer patients.." 2008;62:173-85; discuss. Pubmed PMID: 18626200
- Li Y, Dang TA, Man TK "Plasma proteomic profiling of pediatric osteosarcoma.." Methods Mol. Biol.. 2012;818:81-96. Pubmed PMID: 22083817
- Man TK, Yang JS, Stormo GD "Quantitative modeling of DNA-protein interactions: effects of amino acid substitutions on binding specificity of the Mnt repressor.." Nucleic Acids Res.. 2004;32(13):4026-32. Pubmed PMID: 15289576
- Harris ZL, Durley AP, Man TK, Gitlin JD "Targeted gene disruption reveals an essential role for ceruloplasmin in cellular iron efflux.." Proc. Natl. Acad. Sci. U.S.A.. 1999 Sep 14;96(19):10812-7. Pubmed PMID: 10485908
Projects
- Identification of blood-based biomarkers for ostesosarcoma
- CPRIT
- Examination of the prognostic significance of p27 in osteosarcoma
Memberships
- Children's Oncology Group
- Associate Member
- American Assoication for Cancer Research
- Member
Funding
- Harnessing P27 for Prognostication of Pediatric Osteosarcoma - #1R01HD074553 (01/01/2013 - 12/31/2015) Grant funding from NIH/NICHD
- Targeted Therapies for Metastatic Osteosarcoma - #RP140022-P2 (08/01/2010 - 02/29/2016) Grant funding from Cancer Prevention and Research Institute of Texas
- Targeting the CXCL10-CXCR3 axis in metastatic osteosarcoma
- $1,200,000.00 (08/31/2020 - 08/30/2024) Grant funding from Cancer Prevention and Research Institute of Texas
Skills
- Bioinformatics
- Cancer Research
- Genomics and Proteomics
Languages
Chinese (Cantonese)
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