The Leica SME Laser Scanning Confocal Microscope images 4 channels with excitation wavelengths of 405, 488, 563, and 633 nm. Channels are sequentially imaged using a tunable emission filter. The system is capable of automated tiling for multiple fields or image stacks as well as collection of time series data.
This scope has a fully motorized stage, which facilitates both the image tiling functions as well as the automated collection of z-stacks. Image brightness, background intensity, and focal plane, are all controlled through a digital console behind the keyboard.
This scope is integrated into an image analysis workstation that consists of a DELL Optiplex GX620 processor with a Radeon X600SE video card, Adobe Photoshop image processing software and ImagePro 10.0 image analysis software. The image analysis software consists of a basic analysis module with plug-in modules for image deconvolution (Sharp Stack Plus Ver 5.0), and 3 dimensional reconstruction (3D Constructor).