Overview
The Cancer Prevention and Research Institute (CPRIT) of Texas is supporting a Comprehensive Epigenomics Cancer Core Facility that provides consultation, instrumentation, technical support and data analysis for cancer research projects. The facility operates as a fee-for-service core with very competitive pricing subsidized by the CPRIT grant. The core facility accepts requests for cancer related research from Baylor College of Medicine faculty as a priority.
Services
The core facility provides the following technology platforms as services. Detailed protocols for each platform and policies for operation can be found with attached links:
Single-Cell Genomics Applications
Single-cell analyses include single-cell RNA-seq and single-cell ATAC-seq primarily with the 10x Chromium system. This work is conducted under the direction of Drs. Mira Jeong and Chuck Zong.
“Bulk” Genomics Applications
Epigenomic applications include ATAC-seq, CUT&RUN, DNA methylation profiling and RNA modification profiling.
Spatial Transcriptomics
Spatial analyses can be conducted on various platforms including the 10x Visium, 10x Xenium, Curio Seeker and MERSCOPE. Upstream sample preparation including tissue sectioning, tissue embedding, immunohistochemistry, tissue microarray development, high-resolution imaging and slide scanning are conducted under the direction of Dr. Patricia Castro.
Multi-Omics Data Analysis
Consultation on design of projects and data analysis are also provided as Core Services. Data analysis is performed with customized software tools developed for each of the three technology platforms.
Project Requirements
All projects will require submission of a two-page request for project (RFP) application that will be reviewed for feasibility, appropriateness for the available technology platforms, cancer/epigenomics relevance and scientific merit. Approved RFPs will be prioritized, scheduled accordingly, and specifics of each project will be designed prior to initiation. The PI of the requesting lab is required to attend the initial planning meeting of the approved project. Pricing for projects will be provided upon request. At the completion of each project an additional consultation will be provided to review the analyzed data.
Publications that result from the work of the CPRIT Core facility require acknowledgement of the CPRIT Core Facility grant (Comprehensive Cancer Epigenomics Core Facility; RP RP250580).
Leader and Core Director:
Principle Investigator and Core Director:
Daniel Kraushaar, Ph.D.
kraushaa@bcm.edu
Single Cell Genomics
Leader: Chuck Zong, Ph.D.
chenghang.zong@bcm.edu
Co-Leader: Mira Jeong, Ph.D.
mjeong@bcm.edu
Tissue Processing
Leader: Castro Patricia, Ph.D.
pcastro@bcm.edu
Multi-Omics Data Analysis
Leader: Christian Coarfa, Ph.D.
coarfa@bcm.edu
