CryoEM Core's Major Equipment
The CryoEM Core is a state-of-the-art resource for near-atomic resolution 3-D analysis of the structure and dynamics of macromolecules and assemblies, either purified or within cells. Four Gatan cryo-specimen holders for three of the JEOL 200 kV electron microscopes. Equipment includes:
- One Jeol 60 degree tilt cryo-specimen holder
- Two Jeol 70 degree tilt cryo-specimen holders
- One Jeol 80 degree tilt cryo-specimen holder
- Three Room Temperature specimen holders
All are outfitted to operate in our JEOL 2010f, 2100, and 2200 Microscopes and operate with appropriate cryo-transfer systems.
One FEI Mark IV Vitrification Robot used for cryo-specimen preparation.
One Leica EMGP automatic plunge freezing apparatus for cryo-specimen preparation.
One Fischione Model 1070 Nanoclean, one Ernest Fullam Glow Discharge, and one PELCO easiGlow™ for TEM grid preparation and treatment.
One EMITech carbon evaporators for advanced grid treatment.
One light microscope.
Various Cole Parmer cryo-storage dewars for cryo-specimen and grid preservation and storage.
Tissue culture laboratory equipped with NuAire Biological Safety Cabinet Class II Type A/B3 Model Nu-425-400, NuAire Autoflow IR Direct Heat CO2 Incubator (2x), VWR Fridge (Full Size and Mini), VWR Liquid Nitrogen Storage. Other relevant equipment will be procured soon.
One Aquilos Cryo-FIB: The Thermo Scientific™ Aquilos™ Cryo-FIB is the first cryo-DualBeam™ (focused ion beam/scanning electron microscope) system dedicated to preparation of frozen, thin lamella samples from biological specimens for high-resolution tomographic imaging in a cryo-transmission electron microscope (cryo-TEM).
Our associated laboratories are also equipped for buffer preparation, tissue culture preparation, cell culture preparation, and protein purification. Our ÄKTA Pure HPLC is available for on-site size exclusion chromatography.
Microscopes
The following microscopes are available for use in the CryoEm Core.
Glacios
Glacios Cryo Transmission Electron Microscope (Cryo-TEM) features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot), and the same innovative automation for ease of use.
JEOL3200FSC
JEM3200FSC (300kV) electron microscope with a field emission gun, an in-column energy filter, a cryo-chuck holding up to three grids, a goniometer tilt range (+/-70° tilts) with dual rotation cartridge, a Gatan K2 Summit direct electron detector, SerialEM for automated single particle and tomography data collection.
JEOL2200FS
JEM2200FS (200 kV) electron microscope with a field emission gun, an in-column energy filter, a Zernike phase plate holder for two discs through an airlock exchanges at objective and intermediate lens areas, a 5120 x 3840 pixels Direct Electron Detector camera (DE20), a Gatan 4k x 4k pixels slow scan CCD camera (US4000), SerialEM for automated single particle and tomography data collection.
JEOL2100
JEM 2100 (200 kV) electron microscope with a LaB6 filament, a 3k x 4k Direct Electron Detector camera (DE12), a Gatan 4k x 4k slow scan CCD camera (US4000), and a TEMCon computer interface for automated data collection of single particle with JADAS and cell tomography with SerialEM.
JEOL1230
The JEOL-1230 microscope is a 120 kV instrument with a 4k x 4k CCD camera, and is intended for imaging fixed sections and specimens in negative stain. It is not used for CryoEM. Training is available as a separate service, and is required for all new users of the instrument. After training the instrument is available for individual use at a flat hourly rate. You may also request imaging services from core staff on this instrument.
Service Scheduling
All services are scheduled and tracked using iLab. Click to view scheduling information.
