Mouse Monoclonal Antibody Production


Expression of Monoclonal Antibodies (Mabs) from Existing Hybridomas (In Vitro)

Expression of Mabs from hybridoma cell lines is provided in spinner vessel cell cultures (0.5 to 3 liters) in medium containing low bovine IgG serum or in chemically defined serum-free medium.   Culture supernatants from spinner vessels produce Mabs in the range of 5-50 mg/liter.  Larger amounts of Mab can be produced in CL-1000 Celline flasks with the ability to generate antibody in the range of 50 -300 mg/liter. 

Purification of Monoclonal Antibodies  

MAbs expressed from hybridomas in culture are typically purified by Protein G/A affinity chromatography but can also be purified by custom ion exchange and size exclusion chromatography procedures.  Analysis and Q/C of purified Mabs is provided by SDS-PAGE for purity, protein concentration by Nanodrop and analytic size exclusion chromatography to determine homogeneity of native molecular size.   Endotoxin-free purified Mab can be provided upon request.