Expression of Monoclonal Antibodies (Mabs) from Existing Hybridomas (In Vitro)
Expression of Mabs from hybridoma cell lines is provided in spinner vessel cell cultures (0.5 to 3 liters) in medium containing low bovine IgG serum or in chemically defined serum-free medium. Culture supernatants from spinner vessels produce Mabs in the range of 5-50 mg/liter. Larger amounts of Mab can be produced in CL-1000 Celline flasks with the ability to generate antibody in the range of 50 -300 mg/liter.
Purification of Monoclonal Antibodies
MAbs expressed from hybridomas in culture are typically purified by Protein G/A affinity chromatography but can also be purified by custom ion exchange and size exclusion chromatography procedures. Analysis and Q/C of purified Mabs is provided by SDS-PAGE for purity, protein concentration by Nanodrop and analytic size exclusion chromatography to determine homogeneity of native molecular size. Endotoxin-free purified Mab can be provided upon request.