Research

Protein and Monoclonal Antibody Production Core

Master
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About the Core

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The protein and monoclonal antibody production core is a shared research resource to provide quality purified proteins, monoclonal antibodies, and protein characterization services.

These services includes the protein expression and purification in E.coli, insect and mammalian systems. The core has more than 30 years of excellent experience in producing new mouse monoclonal antibodies using intact proteins, synthetic peptides and subcellular fraction as antigens. Protein characterization services will be offered beginning January 2022.

All core services can be requested in iLab.

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Free Consultation

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The core provides free consult on generating new mouse monoclonal antibody, protein production and protein characterization.

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Recombinant Protein Expression

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The core has large refrigerated shaking incubators and bioreactors to overexpress recombinant proteins of interest in E.coli, insect and mammalian systems.

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Protein and Antibody Chromatography Purification

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The core implements chromatography techniques to purify protein and Mab produced in different expression systems. Quality control and biophysical characterization of purified proteins and MAbs can be performed, including endotoxin and nucleases testing, analytical gel filtration, and Thermofluor/DSF assays.

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Mouse Monoclonal Antibody Production

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The core can generate custom mouse monoclonal antibodies (MAbs), including those targeted to proteins, synthetic peptides and subcellular fractions.

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Protein Characterization

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The core providea various protein characterization technologies and instruments. Instruments are available in the core to characterize proteins and other macromolecules.

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Getting Started

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If you plan to make use of the core, we require a brief (no cost) consultation with our directors. Email atc-protein-mab@bcm.edu for questions or to get set up for a consultation.

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