The cyclin T1 subunit of the HIV Tat cofactor TAK/P-TEFb is transiently induced during macrophage differentiation.
About the Lab
The Rice laboratory studies how gene expression is regulated in HIV - the virus that causes AIDS. The major focus of our research is on the HIV Tat protein, which is essential for HIV transcription, and on two cellular factors, cyclin T1 and Cdk9, which are necessary for Tat function. We are investigating how cyclin T1 and Cdk9 are regulated in the two cell types that HIV infects - CD4+ T cells and macrophages.
On this web site, you will find short summaries of several research projects that are ongoing in the laboratory, information about members of the lab, and some of our laboratory protocols.
Data
Microarray data - From TL Sung, L Golebiewski, E Siwak, and AP Rice. (Manuscript submitted).
Sung TL, Rice AP. PLoS Pathog. 2009 Jan;5(1):e1000263. Epub 2009 Jan 16. miR-198 inhibits HIV-1 gene expression and replication in monocytes and its mechanism of action appears to involve repression of cyclin T1.
Microarray data - from TL Sung and AP Rice. (2006) Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+ T cells. - Retrovirology. 3(1):66.
Microarray data - Yu, W., Wang, Y., Shaw, C., Qin, X.-F., and Rice, A.P. (2006) Induction of HIV-1 Tat co-factor cyclin T1 during monocyte differentiation is required for the regulated expression of a large portion of cellular mRNAs. Retrovirology 3:32.
Microarray data - from R.E. Haaland, W. Yu, and A.P. Rice. (2005) Identification of LKLF-regulated genes in quiescent CD4+ T lymphocytes - Molecular Immunology, 42:627-641.
Microarray data - from Haaland, R.E., Herrmann, C.H., and Rice, A.P. (2005). siRNA depletion of 7SK snRNA induces apoptosis but does not affect expression of the HIV-1 LTR or P-TEFb-dependent cellular genes. J. Cell. Physiol. 205:463-470.
Resources
- Texas Medical Center
- Entrez PubMed
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases
- Office of AIDS Research, NIH
- NIH AIDS Research Reference and Reagent Program*
- American Type Culture Collection
*The GST-Tat constructs made in our lab are available through this program.
