Marco De Giorgi, Ph.D.

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Marco De Giorgi, Ph.D.

Assistant Professor

Email

giorgi@bcm.edu

Positions

Assistant Professor: Integrative Physiology
Baylor College of Medicine
Houston, Texas United States

Education

PhD from Medical University of Gdansk: 10/2016 - Gdansk, Poland

MSc from University of Milano-Bicocca: 02/2012 - Monza, Italy

BSc from University of Salento: 10/2006 - Lecce, Italy

Professional Interests

Gene therapy
Genome editing
Lipid metabolism
Atherosclerosis
Genetic engineering

Selected Publications

De Giorgi M et al "In vivo expansion of gene-targeted hepatocytes through transient inhibition of an essential gene." bioRxiv. 2023 Jul 29; Pubmed PMID: 37546995
De Giorgi M, Jarrett KE, Vallim TA, Lagor WR "In Vivo Gene Editing in Lipid and Atherosclerosis Research." Atherosclerosis. Methods Mol Biol.. 2022 Jan;2419 Pubmed PMID: 35237996
De Giorgi M, Li A, Hurley A, Barzi M, Doerfler AM, Cherayil NA, Smith HE, Brown JD, Lin CY, Bissig K-D, Bao G, Lagor WR "Targeting the Apoa1 locus for liver-directed gene therapy." Mol. Ther. Methods Clin. Dev.. 2021 Jun 11;21:659-669.
Doerfler AM, Park SH, M. Assini JM, Youssef A, Saxena L, Yaseen AB, De Giorgi M, Chuecos M, Hurley AE, Li A, Marcovina SM, Bao G, Boffa MB, Koschinsky ML, Lagor WR "LPA disruption with AAV-CRISPR potently lowers plasma apo(a) in transgenic mouse model: A proof-of-concept study." Mol Ther Methods Clin Dev. 2022 Dec 8;8(27):337-351. Pubmed PMID: 36381302

Wang Y, Hurley A, De Giorgi M, Tanner MR, Hu R, Pennington MW, Lagor WR, Beeton C "Adeno-Associated virus 8 delivers an immunomodulatory peptide to mouse liver more efficiently than to rat liver." PLos One. 2023 Apr 11;18 Pubmed PMID: 37040361
Doerfler AM, Han J, Jarrett KE, Tang L, Jain A, Saltzman A, De Giorgi M, Chuecos M, Hurley A, Li A, Morand P, Ayala C, Goodlett DR, Malovannaya A, Martin JF, Vallim TA, Shroyer N, Lagor WR. "Intestinal Deletion of 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Promotes Expansion of the Resident Stem Cell Compartment." Arterioscler Thromb Vasc Biol.. 2022 Apr;42(4):381-394. Pubmed PMID: 35172604
De Giorgi M, Jarrett KE, Burton JC, Doerfler AM, Hurley A, Li A, Hsu RH, Furgurson M, Patel KR, Han J, Borchers CH, Lagor WR "Depletion of essential isoprenoids and ER stress induction following acute liver-specific deletion of HMG-CoA reductase." J Lipid Res. 2020 Dec;61(12):1675-1686. Pubmed PMID: 33109681
Pastore N, Huynh T, Herz NJ, Calcagni' A, Klisch TJ, Brunetti L, Kim KH, De Giorgi M, Hurley A, Carissimo A, Mutarelli M, Aleksieva N, D'Orsi L, Lagor WR, Moore DD, Settembre C, Finegold MJ, Forbes SJ, Ballabio A "TFEB Regulates Murine Liver Cell Fate During Development and Regeneration." Nat Commun. 2020 May 18; Pubmed PMID: 32424153
Li A, Tanner MR, Lee CM, Hurley AE, De Giorgi M, Jarrett KE, Davis TH, Doerfler AM, Bao G, Beeton C, Lagor WR "AAV-CRISPR Gene Editing Is Negated by Pre-existing Immunity to Cas9." Mol Ther. 2020 Apr 19; Pubmed PMID: 32348718
Li Z, Votava JA, Zajac GJM, Nguyen JN, Leyva Jaimes FB, Ly SM, Brinkman JA, De Giorgi M, Kaul S, Green CL, St Clair SL, Belisle SL, Rios JM, Nelson DW, Sorci-Thomas MG, Lagor WR, Lamming DW, Eric Yen CL, Parks BW "Integrating Mouse and Human Genetic Data to Move beyond GWAS and Identify Causal Genes in Cholesterol Metabolism." Cell Metab.. 2020 Mar 17;pii: S1550-4131(20)30074-7 Pubmed PMID: 32197071
De Giorgi M, Lagor WR "Gene Delivery in Lipid Research and Therapies." Methodist Debakey Cardiovasc J.. 2019;15(1):62-69. Pubmed PMID: 31049151
Li A, Lee CM, Hurley AE, Jarrett KE, De Giorgi M, Lu W, Balderrama KS, Doerfler AM, Deshmukh H, Ray A, Bao G, Lagor WR "A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing." Mol Ther Methods Clin Dev.. 2018 Dec 6;pii: S1525-0016(20)30198-2 Pubmed PMID: 30619914
Jarrett KE, Lee C, De Giorgi M, Hurley A, Gillard BK, Doerfler AM, Li A, Pownall HJ, Bao G, Lagor WR "Somatic Editing of Ldlr With Adeno-Associated Viral-CRISPR Is an Efficient Tool for Atherosclerosis Research." Arterioscler Thromb Vasc Biol. 2018 Jul 19; Pubmed PMID: 30026278
1. Scagliotti A, Capizzi L, Cazzaniga ME, Ilari A, De Giorgi M, Cordani N, Gallazzi M, Bruno A, Pelosi G, Albini A, Lavitrano M, Grassilli M and Cerrito MG "Co-targeting triple-negative breast cancer cells and endothelial cells by metronomic chemotherapy inhibits cell regrowth and migration via downregulation of the FAK/VEGFR2/VEGF axis and autophagy/apoptosis activation." Front Oncol .. 2022 Nov 30; Pubmed PMID: 36531071
Cerrito MG, De Giorgi M, Pelizzoni D, Bonomo SM, Digiacomo N, Scagliotti A, Bugarin C, Gaipa G, Grassilli E, Lavitrano M, Giovannoni R, Bidoli P and Cazzaniga ME "Metronomic combination of Vinorelbine and 5-Fluorouracil inhibit triple-negative breast cancer cells results from the proof-of-concept VICTOR-0 study." Oncotarget. 2018;9(44):27448-27459. Pubmed PMID: 29937997
Romano G, Reggi S, Kutryb-Zajac B, Facoetti A, Chisci E, Pettinato M, Giuffrè MR, Vecchio F, Leoni S, De Giorgi M, Avezza F, Cadamuro M, Crippa L, Leone BE, Lavitrano M, Rivolta I, Barisani D, Smolenski RT, Giovannoni R. "APOA-1Milano muteins, orally delivered via genetically modified rice, show anti-atherogenic and anti-inflammatory properties in vitro and in Apoe-/- atherosclerotic mice." Int J Cardiol. 2018 Jun 11; Pubmed PMID: 29907443
De Giorgi M, Enjyoji K, Jiang G, Csizmadia E, Mitsuhashi S, Gumina JG, Smolenski RT and Robson SC "Complete deletion of Cd39 is atheroprotective in apolipoprotein E-deficient mice." J Lipid Res. 2017;58:1292-1305. Pubmed PMID: 28487312
Chisci E*, De Giorgi M*, Zanfrini E, Testasecca A, Brambilla E, Cinti A, Farina L, Kutryb-Zajac B, Bugarin C, Villa C, Grassilli E, Combi R, Gaipa G, Cerrito MG, Rivolta I, Smolenski RT, Lavitrano M and Giovannoni R "Simultaneous overexpression of human E5NT and ENTPD1 protects porcine endothelial cells against H2O2-induced oxidative stress and cytotoxicity in vitro." Free Radic Biol Med. 2017;108:320-333. Pubmed PMID: 28389406
Baggio Savio LE, De Giorgi M, Robson SC "Ectonucleotidases in Immunobiology." Encyclopedia of Immunobiology. 2016;2:424–431.
Cinti A, De Giorgi M, Chisci E, Arena C, Galimberti G, Farina L, Bugarin C, Rivolta I, Gaipa G, Smolenski RT, Cerrito MG, Lavitrano M, Giovannoni R "Simultaneous overexpression of functional human HO-1, E5NT and ENTPD1 protects murine fibroblasts against TNF-α-induced injury in vitro." Plos One. 2015;10:e0141933. Pubmed PMID: 26513260
De Giorgi M, Cinti A, Pelikant-Malecka I, Chisci E, Lavitrano M, Giovannoni R, Smolenski RT "Co-expression of functional human heme oxygenase 1, ecto-5’-nucleotidase and ecto-nucleoside triphosphate diphosphohydrolase-1 by “self-cleaving” 2A peptide system." Plasmid. 2015;79:22-29. Pubmed PMID: 25779031
De Giorgi M, Pelikant-Malecka I, Sielicka A, Slominska EM, Giovannoni R, Cinti A, Cerrito MG, Lavitrano M, Smolenski RT "Functional analysis of expression of human ecto-nucleoside triphosphate diphosphohydrolase-1 and/or ecto-5’-nucleotidase in pig endothelial cells." Nucleosides Nucleotides Nucleic Acids. 2014;33:313-318. Pubmed PMID: 24940685
4. Walkey CJ, Snow KJ, Bulcha J, Cox AR, Martinez AE, Ljungberg MC, Lanza DG, De Giorgi M et al "Comprehensive Atlas of AAV Tropism in the Mouse." bioRxiv. 2024 Sep 10; Pubmed PMID: 37546995

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Funding

In vivo expansion of gene-targeted hepatocytes for liver gene therapy - #PNC24-260845

Intellectual Property

SELECTIVE EXPANSION OF GENE-TARGETED CELLS: Method Patent #WO/2021/108269; Co Inventors: LAGOR, William HURLEY, Ayrea JARRETT, Kelsey BISSIG, Karl-Dimiter DE GIORGI, Marco FURGURSON, Mia