Email

sludtke@bcm.edu

Positions

Professor

Biochemistry and Molecular Biology
Baylor College of Medicine
Houston, TX US
Primary Appointment

Charles C. Bell Professor

Biochemistry and Molecular Biology
Baylor College of Medicine

Director

CryoEM Core
Baylor College of Medicine

Co-Director

Center for Computational and Integrative Biomedical Research (CIBR)
Baylor College of Medicine
Houston, Texas United States

Member

Dan L Duncan Comprehensive Cancer Center
Baylor College of Medicine

Addresses

Office (Office)

Baylor College of Medicine
BCMN-N420
Houston, TX 77030
United States
Phone: (713) 798-9020
sludtke@bcm.edu

Education

PhD from Rice University

06/1996 - Houston, Texas United States

Physics

MA from Rice University

06/1993 - Houston, Texas United States

Physics

BS from Caltech

06/1990 - Pasadena, California United States

Physics

Honors & Awards

Burton Medal

Microscopy Society of America (01/2008)

HAMBP (Houston Area Molecular Biophysics Program) Fellow

Houston Area Molecular Biophysics Program (01/1992 - 01/1996)

NRSA (Institutional) Fellow

NRSA (01/1996 - 01/1997)

NRSA (Individual) Fellow

NRSA (01/1998 - 01/1999)

Professional Interests

• Cryo-electron Microscopy and Tomography
• Quantitative Image Processing Algorithm Development
• Structural Biology and Macromolecular Assemblies
• Membranes and Membrane Proteins
• Protein Structure-Function and Protein Folding
• Antibiotic Resistance

Professional Statement

My group focuses on CryoEM/CryoET and quantitative image processing, applying these techniques to a wide range of biological systems ranging from individual macromolecules to whole cells. For the last 20 years my group has been developing the EMAN software suite for both high resolution single particle analysis as well as in-situ and in-vitro tomography. This software has been used on a large fraction of the structures in the EMDatabank, and with over 30,000 downloads is one of the most widely used in the field. In addition to developing new image processing methods such as deep learning applications and bispectral analysis, my group engages in a wide range of biological projects, from the high resolution structure and dynamics of individual molecules to the study of functioning macromolecular assemblies within the cell. Over the last 3-5 years, cryoEM has experienced a renaissance, largely due to the development of revolutionary direct detector technology, making this field directly competitive with X-ray crystallography for high resolution structural analysis, and providing many unique capabilities, such as in-situ structural biology. This is an exciting time for the cryoEM field, with many opportunities to substantially advance our fundamental understanding of biomolecular interactions.

Websites

Videos

Selected Publications

• Kim SJ, Fernandez-Martinez J, Nudelman I, Shi Y, ..., Ludtke SJ, Akey CW, Chait BT, Sali A, Rout MP "Integrative structure and functional anatomy of a nuclear pore complex." Nature. 2018;555(7697):475-482. Pubmed PMID: 29539637
• Chen M, Dai W, Sun SY, Jonasch D, He CY, Schmid MF, Chiu W, Ludtke SJ "Convolutional neural networks for automated annotation of cellular cryo-electron tomograms." Nature Meth.. 2017;14(10):983-985. Pubmed PMID: 28846087
• Yi P, Wang Z, Feng Q, Chou CK, Pintilie GD, Shen H, Foulds CE, Fan G, Serysheva I, Ludtke SJ, Schmid MF, Hung MC, Chiu W, O'Malley BW "Structural and Functional Impacts of ER Coactivator Sequential Recruitment." Mol. Cell. 2017;67(5):733-743. Pubmed PMID: 28844863
• J.M. Bell, M. Chen, P.R. Baldwin & S.J. Ludtke "High Resolution Single Particle Refinement in EMAN2.1." Methods. 2016;100:25-34. Pubmed PMID: 26931650

Memberships

Biophysical Society

Member (01/1992)

Microscopy Society of America

Member (01/1998)