CryoEM Core's Major Equipment
The CryoEM Core is a state-of-the-art resource for near-atomic resolution 3-D analysis of the structure and dynamics of macromolecules and assemblies, either purified or within cells. Four Gatan cryo-specimen holders for three of the JEOL 200 kV electron microscopes. Equipment includes:
- One Jeol 60 degree tilt cryo-specimen holder
- Two Jeol 70 degree tilt cryo-specimen holders
- One Jeol 80 degree tilt cryo-specimen holder
- Three Room Temperature specimen holders
All are outfitted to operate in our JEOL 2010f, 2100, and 2200 Microscopes and operate with appropriate cryo-transfer systems.
One FEI Mark IV Vitrification Robot used for cryo-specimen preparation.
One Leica EMGP automatic plunge freezing apparatus for cryo-specimen preparation.
One Fischione Model 1070 Nanoclean, one Ernest Fullam Glow Discharge, and one PELCO easiGlow™ for TEM grid preparation and treatment.
One EMITech carbon evaporators for advanced grid treatment.
One light microscope.
Various Cole Parmer cryo-storage dewars for cryo-specimen and grid preservation and storage.
Tissue culture laboratory equipped with NuAire Biological Safety Cabinet Class II Type A/B3 Model Nu-425-400, NuAire Autoflow IR Direct Heat CO2 Incubator (2x), VWR Fridge (Full Size and Mini), VWR Liquid Nitrogen Storage. Other relevant equipment will be procured soon.
One Aquilos Cryo-FIB: The Thermo Scientific™ Aquilos™ Cryo-FIB is the first cryo-DualBeam™ (focused ion beam/scanning electron microscope) system dedicated to preparation of frozen, thin lamella samples from biological specimens for high-resolution tomographic imaging in a cryo-transmission electron microscope (cryo-TEM).
Our associated laboratories are also equipped for buffer preparation, tissue culture preparation, cell culture preparation, and protein purification. Our ÄKTA Pure HPLC is available for on-site size exclusion chromatography.
The following microscopes are available for use in the CryoEm Core.