Illumina’s iSeq, NextSeq and NovaSeq systems' powerful combination of read-length and paired-end flexibility enables the broadest range of genomic sequencing applications. Illumina's robust sequencing chemistry supports a wide range of read lengths, allowing researchers to tailor each run to meet their needs with shorter turnover times.
| Instrument | iSeq 100 Sequencer | NextSeq 500 Sequencer | NextSeq 500 Sequencer | NovaSeq 6000 Sequencer | NovaSeq 6000 Sequencer | NovaSeq 6000 Sequencer | NovaSeq 6000 Sequencer |
|---|---|---|---|---|---|---|---|
| Mode | Regular | Mid Output | High Output | SP FC | S1 FC | S2 FC | S4 FC |
| Output (Max.) | 1.2 Gb - 2x150bp | 32.5-39 Gb - 2x150bp 16.25-19.5 Gb - 2x75bp | 100-120 Gb - 2x150bp 50-60 Gb - 2x75bp 25-30 Gb - 1x75bp | 400 Gb - single FC | 500 Gb - single FC |
1250 Gb - single FC
| 3000 Gb - single FC |
| Run Time | 17.5 hours | 15 - 26 hours | 11 - 29 hours | 13 - 38 hours | 13 - 25 hours | 16 - 36 hours | 45 hours |
| Single End Reads (Max.) | Up to 4 Million | Up to 130 Million
| Up to 400 Million
| 800 Million | 1.6 Billion | 4.1 Billion | 10 Billion |
| Paired End Reads (Max.) | Up to 8 Million | Up to 260 Million | Up to 800 Million | 1.6 Billion | 3.2 Billion | 8.2 Billion | 20 Billion |
| Read Length (Max.) | 2 x 150 bp | 2 x 150 bp | 2 x 150 bp | 2 x 250 bp | 2 x 150 bp | 2 x 150 bp | 2 x 150 bp |
| Bases above Q30 | > 80% | >75% (2 x 150 bp) >80% (2 x 75 bp)
| >75% (2 x 150 bp) >80% (2 x 75 bp) >80% (1 x 75 bp) | ≥85% (2 x 50 bp) ≥75% (2 x 150 bp) ≥75% (2 x 250 bp) | ≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp) | ≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp) | ≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp) |
Gb = gigabases, bp = base pair
1 Flow Cell (FC) = 8 Lanes
For more information about these instruments see:
- iSeq 100 Sequencer
- NextSeq 500 Sequencer
- NovaSeq 6000 Sequencer
