Master
Media Component
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About the Core
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The Tissue Culture Core Laboratory maintains active cell cultures of many types of mammalian cell lines as well as frozen stocks of a larger cell library deposited by BCM and Molecular and Cellular Biology Department investigators. We also carry stocks of media, reagents, and disposable tissue culture supplies for purchase by core users.
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Services Offered
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- Maintaining Cultured Cell Lines
- Plating of Requested Cells (96 wells up to 15 cm plates)
- Mycoplasma Testing
- STR Analysis Profile (Cell Line DNA Fingerprinting) samples are sent to a collaborating lab
- Training on Culturing & Maintenance of Growing Cell Lines
- Cell Culture Medium (GIBCO) & Custom Specialty Medium
- FBS & Charcoal Stripped FBS
- Reagents & Specialty supplements for media preparation
- Tissue Culture Disposable Plasticware
- Single Cell Cloning
- Primary Cell Culture
- Frozen Cell Vial from our Library
- PBMC and magnetically-isolated cells sourced from blood donors through Gulf Coast Regional Blood Center
- Nucleofection of plasmids or CRISPR/Cas ribonucleoprotein (RNP) complexes for gene editing
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Core Equipment
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- Nikon Bright Field and Florescent Microscope with Camera
- Cell Countess (imaging and cell counter)
- Osmometer
- 100 cubic feet of incubator space
- Four sterile-hood workspaces (BSL2 biosafety cabinets)
- EVOS inverted microscope with fluorescence and digital camera
- Lonza 4D-Nucleofector Core, X, and LV Units
- MultiMACS Cell24 Separator
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Location and Contact
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Baylor College of Medicine, Main Campus
Building: BCM Michael DeBakey Center
Tissue Culture Room: M830
Lab/Office Room: M825
Phone: 713-798-6236
Email: tccmail@bcm.edu
