RNA In Situ Hybridization (ISH)


We perform RNA ISH (mRNA or miRNA) on a high-throughput platform (a Tecan EVO liquid handling robot modified to handle slides). This is a semi-automated liquid handling system modified with a flow-through technology for slide handling and sophisticated temperature control providing automation of approximately 90% of all manual steps in the ISH protocol. All steps of ISH – from pre-hybridization to additions of probes, hybridization, stringency washes and antibody-mediated chromogenic or fluorescent reaction – are carried out by the robotic system. This gives high-quality and reproducible results. We have a 196-slide capacity per run, but most often do 40-96 slides per experiment.


FAQs: In Situ Hybridization