Published Articles
Didenko VV. Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt-Ended DNA Breaks by In Situ Ligation. Methods Mol Biol. 2017;1644:167-177. doi: 10.1007/978-1-4939-7187-9_15. PubMed PMID: 28710763; PubMed Central PMCID: PMC5673075. View publication.
Minchew CL, Didenko VV. Quick Detection of DNase II-Type Breaks in Formalin-Fixed Tissue Sections. Methods Mol Biol. 2017;1644:113-119. doi: 10.1007/978-1-4939-7187-9_9. PubMed PMID: 28710757; PubMed Central PMCID: PMC5654645. View publication.
Didenko VV. Express FRET Labeling and Analysis of Phagocytic Clearance. Methods Mol Biol. 2017;1644:3-11. doi: 10.1007/978-1-4939-7187-9_1. PubMed PMID: 28710749; PubMed Central PMCID: PMC5654643. View publication.
Minchew CL, Didenko VV. Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D. Methods Mol Biol. 2017;1554:229-236. doi: 10.1007/978-1-4939-6759-9_16. PubMed PMID: 28185196; PubMed Central PMCID: PMC5654642. View publication.
Hauser P, Wang S, Didenko VV. Apoptotic Bodies: Selective Detection in Extracellular Vesicles. Methods Mol Biol. 2017;1554:193-200. doi: 10.1007/978-1-4939-6759-9_12. PubMed PMID: 28185192. View publication.
Minchew CL, Didenko VV. Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. PLoS One. 2014 Sep 30;9(9):e108734. doi: 10.1371/journal.pone.0108734. eCollection 2014. PubMed PMID: 25268504; PubMed Central PMCID: PMC4182547. View publication.
Minchew CL, Didenko VV. Assessing phagocytic clearance of cell death in experimental stroke by ligatable fluorescent probes. J Vis Exp. 2014 May 27;(87). doi: 10.3791/51261. PubMed PMID: 24894100; PubMed Central PMCID: PMC4207364. View publication.
Minchew CL, Didenko VV. Selective transport of cationized fluorescent topoisomerase into nuclei of live cells for DNA damage studies. Methods Mol Biol. 2014;1094:167-75. doi: 10.1007/978-1-62703-706-8_14. PubMed PMID: 24162988. View publication.
Didenko VV. Selective detection of phagocytic phase of apoptosis in fixed tissue sections. Methods Mol Biol. 2014;1094:159-66. doi: 10.1007/978-1-62703-706-8_13. PubMed PMID: 24162987. View publication.
Minchew CL, Didenko VV. In vitro assembly of semi-artificial molecular machine and its use for detection of DNA damage. J Vis Exp. 2012 Jan 11;(59):e3628. doi: 10.3791/3628. PubMed PMID: 22258163; PubMed Central PMCID: PMC3353514. View publication.
Minchew CL, Didenko VV. Fluorescent probes detecting the phagocytic phase of apoptosis: enzyme-substrate complexes of topoisomerase and DNA. Molecules. 2011 Jun 3;16(6):4599-614. doi: 10.3390/molecules16064599. PubMed PMID: 21642935; PubMed Central PMCID: PMC3324561. View publication.
Didenko VV. 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach. Methods Mol Biol. 2011;682:77-87. doi: 10.1007/978-1-60327-409-8_7. PubMed PMID: 21057922; PubMed Central PMCID: PMC3324587. View publication.
Didenko VV. In situ ligation simplified: using PCR fragments for detection of double-strand DNA breaks in tissue sections. Methods Mol Biol. 2011;682:65-75. doi: 10.1007/978-1-60327-409-8_6. PubMed PMID: 21057921; PubMed Central PMCID: PMC3324586. View publication.
Hornsby PJ, Didenko VV. In situ ligation: a decade and a half of experience. Methods Mol Biol. 2011;682:49-63. doi: 10.1007/978-1-60327-409-8_5. Review. PubMed PMID: 21057920; PubMed Central PMCID: PMC3324560. View publications.
Didenko VV. In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase. Methods Mol Biol. 2011;682:37-48. doi: 10.1007/978-1-60327-409-8_4. PubMed PMID: 21057919; PubMed Central PMCID: PMC3324588. View publication.
Didenko VV, Minchew CL, Shuman S, Baskin DS. Semi-artificial Fluorescent Molecular Machine for DNA Damage Detection. Nano Lett. 2004 Dec;4(12):2461-6. PubMed PMID: 17330146; PubMed Central PMCID: PMC1805678. View publication.
Didenko VV. Oscillating probe for dual detection of 5'PO4 and 5'OH DNA breaks in tissue sections. Methods Mol Biol. 2006;335:59-69. Review. PubMed PMID: 16785620. View publication.
Didenko VV, Baskin DS. Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots. Biotechniques. 2006 Mar;40(3):295-6, 298, 300-2. PubMed PMID: 16568818; PubMed Central PMCID: PMC1855219. View publication.
Didenko VV, Ngo H, Baskin DS. Polyethyleneimine as a transmembrane carrier of fluorescently labeled proteins and antibodies. Anal Biochem. 2005 Sep 15;344(2):168-73. PubMed PMID: 16095551; PubMed Central PMCID: PMC1853266. View publication.
Didenko VV, Moore VC, Baskin DS, Smalley RE. Visualization of individual single-walled carbon nanotubes by fluorescent polymer wrapping. Nano Lett. 2005 Aug;5(8):1563-7. PubMed PMID: 16089489; PubMed Central PMCID: PMC1853265. View publication.
Baskin DS, Ngo H, Didenko VV. Thimerosal induces DNA breaks, caspase-3 activation, membrane damage, and cell death in cultured human neurons and fibroblasts. Toxicol Sci. 2003 Aug;74(2):361-8. Epub 2003 May 28. PubMed PMID: 12773768; PubMed Central PMCID: PMC1892749. View publication.
Didenko VV, Ngo H, Baskin DS. Early necrotic DNA degradation: presence of blunt-ended DNA breaks, 3' and 5' overhangs in apoptosis, but only 5' overhangs in early necrosis. Am J Pathol. 2003 May;162(5):1571-8. PubMed PMID: 12707041; PubMed Central PMCID: PMC1851179. View publication.
Didenko VV, Ngo H, Minchew CL, Boudreaux DJ, Widmayer MA, Baskin DS. Visualization of irreparable ischemic damage in brain by selective labeling of double strand blunt-ended DNA breaks. Mol Med. 2002 Dec;8(12):818-23. PubMed PMID: 12606816; PubMed Central PMCID: PMC2039967. View publication.
Didenko VV, Ngo H, Minchew CL, Boudreaux DJ, Widmayer MA, Baskin DS. Caspase-3-dependent and -independent apoptosis in focal brain ischemia. Mol Med. 2002 Jul;8(7):347-52. PubMed PMID: 12393932; PubMed Central PMCID: PMC2040004. View publication.
Didenko VV, Ngo H, Baskin DS. In situ detection of double-strand DNA breaks with terminal 5'OH groups. Methods Mol Biol. 2002;203:153-9. PubMed PMID: 12073439. View publication.
Didenko VV. Detection of specific double-strand DNA breaks and apoptosis in situ using T4 DNA ligase. Methods Mol Biol. 2002;203:143-51. PubMed PMID: 12073438. View publication.
Hornsby PJ, Didenko VV. In situ DNA ligation as a method for labeling apoptotic cells in tissue sections. An overview. Methods Mol Biol. 2002;203:133-41. Review. PubMed PMID: 12073437. View publication.
Didenko VV, Ngo HN, Minchew C, Baskin DS. Apoptosis of T lymphocytes invading glioblastomas multiforme: a possible tumor defense mechanism. J Neurosurg. 2002 Mar;96(3):580-4. PubMed PMID: 11883844; PubMed Central PMCID: PMC1853267. View publication.
Didenko VV. Early shape-shifting FRET probe. Biotechniques. 2002 Feb;32(2):272. PubMed PMID: 11848401. View publication.
Didenko VV. DNA probes using fluorescence resonance energy transfer (FRET): designs and applications. Biotechniques. 2001 Nov;31(5):1106-16, 1118, 1120-1. Review. PubMed PMID: 11730017; PubMed Central PMCID: PMC1941713. View publication.
Ding B, Price RL, Goldsmith EC, Borg TK, Yan X, Douglas PS, Weinberg EO, Bartunek J, Thielen T, Didenko VV, Lorell BH. Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure. Circulation. 2000 Jun 20;101(24):2854-62. PubMed PMID: 10859293. View publication.
Didenko VV, Boudreaux DJ, Baskin DS. Substantial background reduction in ligase-based apoptosis detection using newly designed hairpin oligonucleotide probes. Biotechniques. 1999 Dec;27(6):1130-2. PubMed PMID: 10631490; PubMed Central PMCID: PMC1892751. View publication.
Didenko VV, Wang X, Yang L, Hornsby PJ. DNA damage and p21(WAF1/CIP1/SDI1) in experimental injury of the rat adrenal cortex and trauma-associated damage of the human adrenal cortex. J Pathol. 1999 Sep;189(1):119-26. PubMed PMID: 10451498. View publication.
Bozkurt B, Kribbs SB, Clubb FJ Jr, Michael LH, Didenko VV, Hornsby PJ, Seta Y, Oral H, Spinale FG, Mann DL. Pathophysiologically relevant concentrations of tumor necrosis factor-alpha promote progressive left ventricular dysfunction and remodeling in rats. Circulation. 1998 Apr 14;97(14):1382-91. PubMed PMID: 9577950. View publication.
Didenko VV, Tunstead JR, Hornsby PJ. Biotin-labeled hairpin oligonucleotides: probes to detect double-strand breaks in DNA in apoptotic cells. Am J Pathol. 1998 Apr;152(4):897-902. PubMed PMID: 9546350; PubMed Central PMCID: PMC1858232. View publication.
Didenko VV, Hornsby PJ. Presence of double-strand breaks with single-base 3' overhangs in cells undergoing apoptosis but not necrosis. J Cell Biol. 1996 Dec;135(5):1369-76. PubMed PMID: 8947557; PubMed Central PMCID: PMC2121088. View publication.
Didenko VV, Hornsby PJ. A quantitative luminescence assay for nonradioactive nucleic acid probes. J Histochem Cytochem. 1996 Jun;44(6):657-60. PubMed PMID: 8666750. View publication.
Didenko VV, Wang X, Yang L, Hornsby PJ. Expression of p21(WAF1/CIP1/SDI1) and p53 in apoptotic cells in the adrenal cortex and induction by ischemia/reperfusion injury. J Clin Invest. 1996 Apr 1;97(7):1723-31. PubMed PMID: 8601638; PubMed Central PMCID: PMC507237. View publication.
Yang L, Didenko VV, Noda A, Bilyeu TA, Darlington DJ, Smith JR, Hornsby PJ. Increased expression of p21Sdi1 in adrenocortical cells when they are placed in culture. Exp Cell Res. 1995 Nov;221(1):126-31. PubMed PMID: 7589237. View publication.
Meerson FZ, Didenko VV, Arkhipenko IuV, Saltykova VA. [Dynamic expression of the c-myc and Ca-ATPase genes in heart muscle during adaptation to repeated stress]. Biull Eksp Biol Med. 1994 Feb;117(2):124-6. PubMed PMID: 7516741. View publication.
Didenko VV. Biotinylation of DNA on membrane supports: a procedure for preparation and easy control of labeling of nonradioactive single-stranded nucleic acid probes. Anal Biochem. 1993 Aug 15;213(1):75-8. PubMed PMID: 8238885. View publication.
Didenko VV. Polyethylene tube: an alternative support for synthesis and purification of biotin-labeled single-stranded DNA probes. Nucleic Acids Res. 1993 May 11;21(9):2279-80. PubMed PMID: 8502583; PubMed Central PMCID: PMC309513. View publication.
Meerson FZ, Arkhipenko IVb, Rozhitskaia II, Didenko VV, Sazontova TG. [Opposite effects on antioxidant enzymes of adaptation to continuous and intermittent hypoxia]. Biull Eksp Biol Med. 1992 Jul;114(7):14-5. PMID: 1421294. View publication.
Meerson FZ, Didenko VV. [The role of cellular oncogenes in cardiac adaptation to increased load and ischemia]. Kardiologiia. 1992 Feb;32(2):82-90. Review. PubMed PMID: 1527945. View publication.
Evsev'eva MV, Shinkarenko VS, Didenko VV, Meerson FZ. [Limitation of ischemic necrosis by the use of antioxidant ionol]. Biull Eksp Biol Med. 1989 Aug;108(8):152-4. PubMed PMID: 2804318. View publication.
Meerson FZ, Beloshitskiĭ PV, Radzievskiĭ SA, Vorontsova EIa, Didenko VV. [Effect of transauricular electroacupuncture on ischemic and reperfusion arrhythmia under mid-altitude conditions]. Dokl Akad Nauk SSSR. 1989;304(5):1266-8. PubMed PMID: 2737078. View publication.
Arkhipenko IuV, Didenko VV, Sazontova TG, Meerson FZ. [Comparative evaluation of the effect of immobilization stress on the dynamics of resistance to induction of lipid peroxidation in internal organs and brain]. Dokl Akad Nauk SSSR. 1989;304(6):1500-3. PubMed PMID: 2721376. View publication.
Meerson FZ, Arkhipenko IuV, Didenko VV. [Selective suppression of lipid peroxidation in the brain under stress]. Biull Eksp Biol Med. 1988 Nov;106(11):542-4. PubMed PMID: 3196850. View publication.
Ustinova EE, Saltykova VA, Didenko VV, Beloshitskiĭ PV, Meerson FZ. [Effects of adaptation to periodic and continuous hypoxia in disorders of electrical stability of the heart in postinfarction cardiosclerosis]. Biull Eksp Biol Med. 1988 May;105(5):533-5. PubMed PMID: 3382726. View publication.
Didenko VV. [Possible role of lipid peroxidation in the pathogenesis of arrhythmias in myocardial infarct]. Biull Eksp Biol Med. 1985 Jun;99(6):647-9. PubMed PMID: 4016250. View publication.
Savov VM, Didenko VV, Dosmagambetova RS, Meerson FZ, Kagan VE. [Lipid peroxidation in the myocardium in experimental infarct]. Nauchnye Doki Vyss Shkoly Biol Nauki. 1985;(5):30-3. PubMed PMID: 4027287. View publication.
Meerson FZ, Didenko VV, Savov VM, Kopaladze RA, Dosmagambetova RS. [Lipid peroxidation in experimental myocardial infarct: the action of hyperbaric oxygenation]. Biull Eksp Biol Med. 1984 Oct;98(10):398-400. PubMed PMID: 6498314. View publication.
Kagan VE, Savov VM, Didenko VV, Arkhipenko IuV, Meerson FZ. [Relation between the activity of antioxidant systems and endogenous lipid peroxidation in the myocardium of the left and right heart ventricles]. Biull Eksp Biol Med. 1984 Jun;97(6):664-6. PubMed PMID: 6743802. View publication.
Meerson FZ, Saltykova VA, Didenko VV, Savov VM, Kagan VE. [Role of lipid peroxidation in the pathogenesis of arrhythmias and the anti-arrhythmogenic action of antioxidants]. Kardiologiia. 1984 May;24(5):61-8. PubMed PMID: 6748473. View publication.
Kagan VE, Savov VM, Didenko VV, Arkhipenko IuV, Meerson FZ. [Calcium and lipid peroxidation in the heart mitochondrial and microsomal membranes]. Biull Eksp Biol Med. 1983 Apr;95(4):46-8. PubMed PMID: 6831018. View publication.
