Histology and Biochemistry

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Baylor resumes normal business operations on Thursday, Jan. 23

Baylor College of Medicine and its clinics, closed Wednesday, Jan. 22, will reopen under normal business operations on Thursday, Jan. 23. Patient appointments will be as scheduled on Thursday.

Joanna Jankowsky Lab

Master

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Thioflavine plaque

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Triple Labeling for Amyloid, Microglia, and a Therapeutic Anti-Aβ Antibody

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Thioflavine-S staining similar to that shown here was used by student Angie Chiang to measure fibrillar amyloid before and after treatment in our APP transgenic mice. Related Link: Genetic modulation of soluble Aβ rescues cognitive and synaptic impairment in a mouse model of Alzheimer’s disease

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General Use

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Buffers and broths

Dox dosage calculations

Paraformaldehyde for tissue fixation

Transcardial perfusion

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Biochemistry

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Diethylamine extraction for soluble Aß measurement from Liz Eckman

Formic acid extraction for total Aß measurement from Linda and Steve Younkin

Immunoprecipitation of brain proteins

Proteinase K tissue DNA extraction

SDS/Formic acid extraction for Aß measurement from Linda and Steve Younkin

TBS / TBS-X / Guanidine extraction for Aß measurement from Guojun Bu

Western blotting for brain proteins

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Histology

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Aβ immunohistochemistry

Antibodies used by the lab

Campbell-Switzer silver stain

Congo Red stain

Cresyl violet staining

Fractin immunostain

Gallyas silver stain

Gelatin embedding of fixed tissue

Golgi staining with FD Rapid Golgi kit

Hematoxylin and eosin staining

Immunocytochemistry of cultured cells

Immunohistochemistry with free-floating sections

Nuclear fast red stain

Perls Prussian blue stain

RNase pretreatment for nuclear cresyl violet stain

Thioflavine-S staining for amyloid deposits

Timm’s staining

von Kossa calcium stain

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Cell Culture

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HEK cell maintenance

N2a cell maintenance