Master
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The Flow Cytometry Facility provides multi-parameter cellular analysis and viability assessment using state-of-the-art equipment:
- BDIS Canto-I, 2 laser-6 color cytometer
- BDIS Canto-II, 3 laser-8 color cytometer
- BDIS FACScan, 1 laser-3 color cytometer
- BDIS Lyse-Wash Assistent (X3
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Qualifications
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- Accredited by the College of American Pathologists
- CLIA certified as a high-complexity laboratory
- Served as reference laboratory for National Marrow Donor Program
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Staff Experience
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The staff offers experience in:
- Evaluation of CD34+ stem cells in peripheral blood, bone marrow, and umbilical cord blood
- Qualitative and quantitative assessment of specific cellular doses for transplant
- Analysis of the efficacy of cell specific purging and selection procedures
- Immunophenotyping of therapeutic cell populations used in clinical trials
- Evaluation of immune reconstitution of patients following infusion of grafts and therapeutic cell populations
- Assistance in the development and implementation of new cell processing and culture procedures
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Additional Services
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Additional services include:
- Analysis and interpretation of data
- Provide final reports including statistical analysis, graphic and visual interpretation
- Establishment and maintenance of protocol specific databases
- Development of prediction algorithms for cell specific collection, purging and selection procedures
- Provision of training for visiting investigators and technical staff who are establishing stem cell laboratories
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For additional information contact April G. Durett.
