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Trace fluorescence labeling
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Simple 0.1% protein labeling protocol
- Prepare 5 mM stock solution of a succinimidyl ester dye in DMSO
- Add appropriate amount of dye to protein solution for 0.1 % labeling of lysine residues assuming a 1:1 stochiometric labeling efficiency to amine residues
- Wait 5 minutes for 90% dye binding
* There is no need for purification and samples are still fluorescent after 120 days
