Intracytoplasmic sperm injection or ICSI - often referred to as micromanipulation - is used in conjunction with in vitro fertilization (IVF), meaning the eggs are mixed with sperm outside the body.
The procedure starts with retrieving eggs from the patient, then injecting a single sperm directly into each egg to achieve fertilization. The eggs are injected using specially designed microscopes, glass needles and micromanipulation equipment, in a very delicate procedure. The process is repeated for each egg. The eggs are then examined for signs of fertilization while in the incubator (EmbryoScope®) so they may develop into embryos and later be transferred into the uterus.