Listing of Services
HLA Typing
- Methods: RT-PCR, r-SSO, Sequencing
- Low and High Resolution HLA typing of HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQ1A, DQ1B, DPA1, DPB1 loci, E, F, G, H, MICA, MICB, KIR
Antibody Testing
- Flow PRA - HLA antibody screening using flow cytometry
- Single Antigen Beads - HLA antibody detection using Luminex XMap
- C1q antibody testing
Chimerism
- Identification of informative loci
- Detection of Donor and Recipient in various cell populations
Crossmatching
- Complement Dependent Cytotoxicity - serological based B and T cell crossmatch with Anti-Human Immunoglobulin
- 3-Color Flow Crossmatch - Flow cytometric B and T cell crossmatch
Virtual Crossmatching
Paired Kidney Exchange Evaluation
| Test | Tubes to Draw | Turn Around Time |
|---|---|---|
| HLA Typing (A, B, C, DRB1, DRB3,4,5, DQA1, DQB1 DPA1, DPB1) |
1- Acid citrate dextrose Additives (ACD) solution A or 1-(EDTA) purple top |
Five business days; NGS - 10 days |
| PRA / Serum Screen & Antibody ID or Antibody ID titrations |
1- 10cc clot activator- red top | Five business days |
| Crossmatches: Flow Cytometry and CDC |
Recipient: 1- 10cc clot- red top Donor: 4-ACD solution A AutoXM Recipient 4-ACD solution A |
Five business days |
*STAT Testing for all services is 24 hours TAT
Blood Draw or Buccal Swab Instructions
Label the sample tube with the information below. Improper labels will be rejected and discarded. Send the blood sample as soon as it’s drawn overnight with the requisition. Do not send samples on Friday.
- Patient name
- Date of birth, MRN or SSN
- Draw date and phlebotomist’s initial
Test Requisition: contact the lab at HLAclientservices@bcm.edu.
Shipping Address
BCM Immune Evaluation Laboratory
C/O: Peter Jindra
6501 Fannin St., NA 404
Methodist Neurosensory Building
Houston TX 77030
Phone number: (713) 798-3005
Fax number: (713) 798-5576
Sample Drop off instructions:
Samples can be dropped off in the lab, however we prefer all samples are placed in our secure dropbox located to the left of the lab’s front door. Once the sample is dropped off please ring the door-bell to notify us of sample arrival. If this is deceased donor material page the on call technologist.
